Conchita Martinez of Spain became the latest women's seed to fall at Roland Garros when she was defeated 3-6 6-3 6-4 by Zimbabwe's Cara Black.
The demise of eighth-seed Martinez means that no Spanish woman remains in the last 16 of the French Open for the first time since 1986.
America's Meghann Shaughnessy, the number 16 seed, overcame Janette Husarova of Slovakia 7-5 6-4 while Russia's Nadia Petrova battled past Amy Frazier of the United States 6-7 6-4 6-3.