Officials have identified a second area of Zika transmission on the US mainland, Florida governor Rick Scott said.
Five Zika infections have been linked to an area that encompasses most of tourist-friendly South Beach, Mr Scott told reporters.
Officials believe the cases were transmitted by mosquitoes, Florida department of health spokeswoman Mara Gambineri said.
Another infection zone was previously identified in Miami's Wynwood arts district.
Two of the Miami Beach cases involved Miami-Dade County residents, and three involved tourists from New York, Texas and Taiwan, Mr Scott said.
He did not say whether mosquito bites caused the infections.
The county has begun an aggressive mosquito eradication plan in Miami Beach, Mr Scott said.